目前国内制备冰冻红细胞多采用先慢后快的手工方法,添加甘油的速度尚无统一标准,整个添加过程受人为因素影响较大,造成添加甘油时速度不统一、不可控,间接影响冰冻解冻去甘油红细胞的质量。本研究将输液泵进行改造,实现两段程序添加甘油,现将输液泵和手工方法添加甘油制备的冰冻解冻去甘油红细胞进行质量比较,报告如下。材料与方法1.材料(1)血液来源:随机选取本中心4℃保存6 d的30袋悬浮红细胞(2 U/袋),2 600×g,25 min,离心去 At present, the preparation of frozen red blood cells by using more slowly after the first manual method, there is no uniform rate of addition of glycerol, the entire process of adding a greater impact by human factors, resulting in the addition of glycerol speed is not uniform, uncontrollable, indirectly affect the freezing thaw The quality of glycerol red blood cells. This study will transform the infusion pump to achieve two procedures to add glycerin, the infusion pump and manual methods are added glycerol prepared frozen thawing to glycerol red blood cells for quality comparison, the report is as follows. MATERIALS AND METHODS 1. Materials (1) Blood source: Randomly select 30 bags of suspended red blood cells (2 U / bag) stored at 4 ℃ for 6 days at 2 600 × g for 25 min and centrifuged