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采用高效液相色谱法,对野木瓜中齐墩果酸的含量进行了测定。实验表明在色谱柱为Shim-pack VP-ODS柱(250 nm×4.6 mm,5μm)、流动相为乙腈-1.1%乙酸水(946∶)、流速为0.5 mL/min、检测波长205 nm、柱温为室温、进样量20μL时,分离检测效果最佳,齐墩果酸含量在0.03～0.15 mg/mL范围内线性关系良好、精密度高、重现性好,回归方程:y=3.81424×10-8x-0.00240239,相关系数r=0.9994,平均加样回收率为100.54%。实验测得野木瓜中齐墩果酸平均含量为0.1055%。
High-performance liquid chromatography was used to determine the content of oleanolic acid in wild papaya. The experiments showed that the column was a Shim-pack VP-ODS column (250 nm×4.6 mm, 5 μm), the mobile phase was acetonitrile-1.1% acetic acid water (946:), the flow rate was 0.5 mL/min, the detection wavelength was 205 nm, and the column was When the temperature was room temperature and the injection volume was 20 μL, the separation detection effect was the best. The content of oleanolic acid in the range of 0.03 to 0.15 mg/mL had good linearity, high precision, and good reproducibility. The regression equation: y=3.811424× 10-8x-0.00240239, correlation coefficient r = 0.9994, average sample recovery was 100.54%. The average content of oleanolic acid in wild papaya was 0.1055%.