应用ＡＰＡＡＰ方法，Ｄｏｔ－ｂｌｏｔ地高辛标记核酸杂交技术及ＡＢＣ－ＥＬＩＳＡ法分别检测哮喘患儿淋巴细胞ＣＤ２３ｍＲＮＡ，膜ＣＤ２３分子表达和患儿血浆和淋巴细胞诱生ＩＦＮ－γ水平。结果，哮喘患儿ＣＤ２３ｍＲＮＡ表达增高，外周血ＰＢＭＣ和Ｂ细胞的ＣＤ２３表达均显著增加，ＩＦＮ－γ诱生水平明显低下，血清ＩｇＥ水平异常升高，Ｂ细胞ＣＤ２３分子表达与ＩｇＥ水平之间显著正相关，麻疹疫苗治疗后Ｂ细胞ＣＤ２３分子表达明显降低，而ＩＦＮ－γ诱生水平仍低下。 APAAP method, Dot-blot digoxigenin-labeled nucleic acid hybridization and ABC-ELISA were used to detect the expression of CD23mRNA, membrane CD23 and IFN-γin plasma and lymphocyte of asthmatic children. As a result, the expression of CD23 mRNA in asthmatic children was increased, the expression of CD23 in peripheral blood PBMC and B cells were significantly increased, the level of IFN-γ was significantly lower, the level of serum IgE was abnormally elevated, and the positive expression of CD23 between B cells and IgE was significantly positive Related, measles vaccine treatment of CD23 molecules in B cells decreased significantly, while the level of IFN-γ is still low.