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植物名称铁棍山药(Dioscorea sp.)、半枝莲(Scutellaria barbato D.Don)、白毛夏枯草(Azuga decumbens Thunb.)。材料类别铁棍山药和半枝莲均取其嫩枝,常规消毒后切取带节的小段插入培养基,进行诱导培养。白毛夏枯草的茎段经常规消毒后去其表皮后接种。培养条件培养基:(1)MS+2,4-Dlmg/l(激素浓度单位下同)+NAA0.2+BA0.5;(2)MS+NAA0.2+BA2;(3)MS+NAA0.3+BA3+0.5%活性炭;(4)MS+NAA0.2+0.5%活性炭;(5)1/2B_5+NAA0.1。培养温度26±1℃。每日光照12小时,光照度约1500
Plant name Dioscorea sp., Scutellaria barbato D. Don, Azuga decumbens Thunb. Materials Category Iron sticks and Scutellaria barbata take its twigs, routine disinfection cut with small sections inserted into the medium, induction culture. Prickly ash stem section routine disinfection to the epidermis after inoculation. (2) MS + NAA0.2 + BA2; (3) MS + NAA0 (1) MS + 2,4-Dlmg / .3 + BA3 + 0.5% activated carbon; (4) MS + NAA0.2 + 0.5% activated carbon; (5) 1 / 2B_5 + NAA0.1. Culture temperature 26 ± 1 ℃. Daily light 12 hours, illumination of about 1500