近年来对具有喹啉结构的抗疟药(QCDs)的作用机制研究表明,QCDs的靶位为疟原虫的酸性食物泡。作者用酸化(pH4—5.5)的人红细胞溶解物模拟了疟原虫酸性食物泡消化宿主红细胞的过程,并对QCDs的抗疟机制进行了研究。 红细胞取自A型或O型健康供血者,经洗涤离心除去血浆、白细胞和血小板后经低渗溶解或反复冻融的方法制成红细胞溶解 In recent years, the mechanism of action of antimalarial drugs (QCDs) with quinoline structure has been studied, indicating that the targets of QCDs are acidic food vesicles of Plasmodium. The authors used acidified (pH4-5.5) human erythrocytes lysate to simulate the course of digestion of host erythrocytes by malaria parasite acidic food and studied the antimalarial mechanism of QCDs. Erythrocytes from type A or type O healthy donor, washed by centrifugation to remove plasma, white blood cells and platelets by hypotonic dissolution or repeated freeze-thaw method made of red blood cell lysis