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目的通过用EB病毒转化外周血B淋巴细胞使之成为永生细胞株的方法,建立新疆和田维吾尔族长寿老人永生细胞株,为永久保存新疆长寿老人的基因遗传资源及后续研究奠定基础。方法常规制备EB病毒上清,用环孢素法和冻存全血法转化外周血B淋巴细胞。结果以环孢素法成功建立了5株新疆和田维吾尔族长寿老人永生细胞,其中百岁2人,90～99岁3人,建系成功率为100%(5/5),以冻存全血法建系成功1例(1/5)。HLA基因分型结果在建系前后完全一致。结论与冻存全血法相比,环孢素法能更有效地转化长寿老人永生细胞株,可为长寿的分子遗传机制研究以及某些细胞生物学性状的检测提供充足的实验材料。
Objective To establish an immortalized immortalized immortalized human cell line from Xinjiang Uygur by transforming Epstein-Barr virus into peripheral immortalized B lymphocytes and to lay the foundation for the permanent preservation of genetic genetic resources and subsequent research of long-lived human in Xinjiang. Methods The EB virus supernatant was routinely prepared and the peripheral blood B lymphocytes were transformed with ciclosporin and cryopreserved whole blood. Results The 5 immortalized human cells of Uygur longevity elderly were successfully established by cyclosporine method. Among them, 2 were centenarians and 3 were 90-99 years old. The success rate of establishing pedigree was 100% (5/5) French Department of Building a successful case (1/5). HLA genotyping results were exactly the same before and after the establishment of the department. Conclusion Compared with the cryopreserved whole blood method, cyclosporine can more effectively transform immortalized human longevity cell lines, which can provide sufficient experimental materials for the study of molecular mechanism of longevity and the detection of some cell biological traits.