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目的:对在大样本新生儿苯丙酮尿症(PKU)患者中采用荧光分析法进行筛查的效果进行探究分析。方法:将该院2010年4月~2014年4月出生的64 892例新生婴儿作为研究对象,对筛查结果进行探究分析。结果:化学荧光法筛查,PKU筛出率4.62/10万(3/64 892);2周内检测的苯丙氨酸(Phe)含量(0.75±0.25)mg/dl低于1周内检测含量(0.95±0.27)mg/dl,差异具有统计学意义(P<0.05);与振荡5 min相比,振荡10 min、15 min后Phe含量显著增高,差异具有统计学意义(P<0.05),而振荡10 min、15 min后Phe含量间差异无统计学意义(P>0.05)。结论:荧光分析法在大样本新生儿PKU筛查中具有较高的临床应用价值,荧光分析法具有灵敏、稳定等优势,但是需要把握好洗脱时间。
Objective: To investigate the effect of screening by fluorimetric assay in patients with large sample of neonatal phenylketonuria (PKU). Methods: A total of 64 892 newborn infants born from April 2010 to April 2014 in the hospital were studied. The screening results were analyzed. Results: The detection rate of PKU was 4.62 / 100 000 (3/64 892) by chemiluminescence method. The content of Phe in two weeks (0.75 ± 0.25) mg / dl was less than 1 week (P <0.05). Compared with sham 5 min, the content of Phe increased significantly after 15 min of shaking (P <0.05), and the difference was statistically significant (P 0. 05) , While there was no significant difference in Phe content between 10 min and 15 min after shaking (P> 0.05). Conclusion: Fluorescence analysis has high clinical value in the screening of large samples of neonatal PKU. Fluorescence analysis has the advantages of sensitivity and stability, but it needs to grasp the elution time.