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目的 为了明确广东省南海市１９９８ 年夏、秋季一批发烧、出疹病人的诊断。方法收集疑似登革热（ＤＦ）患者血清５２份，应用逆转录聚合酶链反应（ＲＴＰＣＲ）、病毒分离和间接免疫荧光技术分别进行了病原学和血清学检测。结果 从２５ 份发病早期患者血标本中分离病毒１０ 份，经用单克隆抗体间接免疫荧光检测和ＲＴＰＣＲ检测证实ＤＥＮ２ 型感染。ＩｇＧ抗体检测，阳性率为４６－１５％ （２４／５２） ，最高滴度达１∶１２８０，ＩｇＭ 抗体检测阳性率为６０％ （１５／２５）。部分病人双份抗体检测，抗体滴度４ 倍升高。结论 此次南海市登革热暴发流行为登革２ 型感染所致。
Objective To clarify the diagnosis of a group of fever and rash in Nanhai, Guangdong Province in summer and autumn of 1998. Methods 52 serum samples from suspected dengue fever (DF) patients were collected for pathogenic and serological tests using reverse transcription polymerase chain reaction (RTPCR), virus isolation and indirect immunofluorescence. Results Ten samples of virus were isolated from 25 blood samples of patients with early onset. DEN2 infection was confirmed by indirect immunofluorescence assay and RT-PCR with monoclonal antibodies. IgG antibody, the positive rate was 46-15% (24/52), the highest titer was 1:1280, and the positive rate of IgM antibody was 60% (15/25). Some patients double antibody test, antibody titer 4 times higher. Conclusion Dengue outbreak in Nanhai City is caused by dengue type 2 infection.