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目的:探讨Kruippel样转录因子3(Kruippel-like factor 3,KLF3)在肺腺癌中的表达及其与临床特征的关系,为探索KLF3在肺腺癌中的作用提供依据。方法:荧光定量PCR及Western blot分析KLF3的mRNA及蛋白在肺腺癌细胞株中的表达。收集72例肺腺癌患者的肿瘤组织标本,免疫组织化学法分析KLF3在肺腺癌组织中表达并分析KLF3表达与患者临床特征的关系。结果:KLF3在肺腺癌细胞株中表达下调。大部分肺腺癌组织中KLF3低表达(50/72),其表达主要定位于胞浆及胞核内。KLF3表达与肺腺癌临床病理分期(P<0.000 1)及T分期(P=0.004)、N分期(P<0.000 1)、M分期(P<0.000 1)明显相关。结论:KLF3可能是一个重要的肺腺癌抑癌基因,并有望成为新的抗肿瘤治疗靶点。
Objective: To investigate the expression of Kruippel-like factor 3 (KLF3) in lung adenocarcinoma and its relationship with clinical features, and provide basis for exploring the role of KLF3 in lung adenocarcinoma. Methods: The expression of KLF3 mRNA and protein in lung adenocarcinoma cell lines was analyzed by real-time PCR and Western blot. The tumor tissue samples from 72 patients with lung adenocarcinoma were collected. The expression of KLF3 in lung adenocarcinoma was analyzed by immunohistochemistry and the relationship between KLF3 expression and clinical characteristics was analyzed. Results: KLF3 was down-regulated in lung adenocarcinoma cell lines. In most lung adenocarcinoma tissues, KLF3 is lowly expressed (50/72), and its expression is mainly located in the cytoplasm and nucleus. KLF3 expression was significantly correlated with clinical pathological stage (P<0.0001), T stage (P=0.004), N stage (P<0.0001), and M stage (P<0.0001). Conclusion: KLF3 may be an important tumor suppressor gene of lung adenocarcinoma, and it is expected to become a new antitumor therapeutic target.