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用抗水稻白叶枯病的近等基因系CBB3(Xa—3)、CBB4(Xa—4)、CBB12(Xa—12)和感病轮回亲本沈农1033与两个致病力不同的白叶枯病菌株75—1、76—25组成非亲和性和亲和性反应的组合。以无菌水模拟接种为对照,成株期剪叶接种后分别在不同时间取样测定叶片的苯丙氨酸解氨酶(PAL)、过氧化物酶(PO)和超氧化物歧化酶(SOD)活性。PAL活性在处理后48h达到高峰,且接种病菌后的酶活性增长率与品系的抗病性成正相关;病菌和模拟接种都刺激水稻叶片PO活性上升,接种病菌,PO呈双峰曲线,抗病品系比感病品种的上升速度快;接种病菌后抗病品系的SOD活性上升,但很快下降,然后再上升,感病品种则下降,且始终低于模拟接种的活性。
In this study, the two isogenic lines CBB3 (Xa-3), CBB4 (Xa-4), CBB12 (Xa-12) and susceptible parent Shennong 1033 Bacterial blight strains 75-1, 76-25 constitute a combination of non-affinity and affinity reactions. Inoculated with sterilized water as control, the leaf phenylalanine ammonia lyase (PAL), superoxidase (PO) and superoxide dismutase (SOD) were sampled at different time after inoculation, )active. PAL activity peaked at 48h after treatment, and the rate of increase of enzyme activity after inoculation was positively correlated with the disease resistance of the lines. Pathogen and simulated inoculation all stimulated the increase of PO activity in rice leaves, inoculated with bacteria, PO showed bimodal curve, disease resistance The susceptible lines increased faster than the susceptible lines. After inoculated with the pathogen, the SOD activity of resistant lines increased, but decreased rapidly and then increased. The susceptible lines decreased and remained lower than the inoculation activity.