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生物样品折射率的空间变化导致了光学畸变的产生,这种畸变对于共聚焦显微镜观察厚的生物样品和活体体内组织成像是一种严重的限制。自适应光学(AO)技术是通过快速反应的变形镜使镜面发生形变来补偿像差,在共聚焦显微镜中应用自适应光学技术可以校正光学畸变,观察深层组织活动,进行活体成像和实时检测。详细分析了共聚焦显微镜中像差的来源及光学畸变的特点,讨论了目前在共聚焦显微镜中自适应光学校正的方案及研究现状,讨论了共聚焦显微镜中自适应光学的波前传感器、畸变测量和波前校正器,并探讨了目前超高分辨率显微成像技术的发展方向。
The spatial variation of the refractive index of a biological sample results in the generation of optical distortions that are a serious limitation for the confocal microscope observation of thick biological samples and in vivo tissue imaging. Adaptive Optics (AO) compensates for aberrations by deforming the mirror surface with a fast reacting deformable mirror. Adaptive optical techniques are used in confocal microscopy to correct optical distortions, observe deep tissue activity, and perform in vivo imaging and real-time detection. The origin of aberration and the characteristics of optical aberration in the confocal microscope are analyzed in detail. The current scheme and research status of the adaptive optical correction in the confocal microscope are discussed. The adaptive optical wavefront sensor in the confocal microscope is discussed. The distortion Measurement and wavefront corrector, and explores the current development of ultra-high resolution microscopy.