我们用琼脂凝胶免疫电泳和双向扩散试验技术对包虫病患者术前血清进行诊断分析,观察发现考马氏蓝染色前后的阳性率有很大差别,现报告如下。 包虫病人血清63例,55例系手术证实的术前血,8例为临床诊断病人(ID、IHA、ELISA均阳性)。正常人血清共20份,均为健康者血清。用羊肝包虫囊液冻干粉末做为抗原,IEP所用抗原浓度为200mg/ml,DD_5为50mg/ml。IEP和DD_5试验均按常规方法进行。 We use agar gel immunoelectrophoresis and two-way diffusion test of hydatid disease in patients with preoperative serum analysis, found that positive rates before and after the Coomassie blue staining are very different, are as follows. Echinococcosis sera 63 cases, 55 cases of preoperative blood confirmed by surgery, 8 cases were clinically diagnosed patients (ID, IHA, ELISA were positive). A total of 20 normal human serum, are healthy serum. Lyophilized hydatid fluid was used as antigen, IEP antigen concentration was 200mg / ml, DD_5 was 50mg / ml. IEP and DD_5 tests were conducted according to conventional methods.