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该文研究了双相体系中固定β-葡萄糖苷酶水解黄芩苷制备黄芩素的最佳工艺条件并考察其产率,考察了黄芩素在多种有机溶剂中的溶解性及其在水与有机溶剂中的分配系数,确定了固定化酶催化黄芩苷酶解反应在醋酸缓冲液和氯仿组成双相体系中进行。以海藻酸钠为载体,用交联-包埋法固定化β-葡萄糖苷酶,并测定了其最适反应温度,最适反应pH,米氏常数,热稳定性和pH稳定性等酶学性质。比较了固定化酶在双相体系中水解黄芩苷的产率,得出最佳反应条件:pH 5.0的醋酸缓冲液和三氯甲烷组成的双相体系,反应温度50℃,反应时间10 h,黄芩素产率为85.28%。与单相反应体系相比,双相反应体系中反应速度和产率均有所提高。
In this paper, we studied the optimum conditions for the preparation of baicalein by immobilizing β-glucosidase in bi-phasic system and investigated the yield of baicalein. The solubility of baicalein in various organic solvents and its effect on water and organic The partition coefficient of the solvent determined that immobilized enzyme catalyzed the baicalin digestion reaction in a biphasic system composed of acetate buffer and chloroform. Using sodium alginate as carrier, β-glucosidase was immobilized by cross-linking-embedding method and its optimum reaction temperature, optimum pH, Mie constants, thermostability and pH stability were determined nature. The yields of baicalin hydrolyzed by immobilized enzyme in two-phase system were compared. The optimum reaction conditions were as follows: the biphasic system consisting of acetic acid buffer and chloroform at pH 5.0, the reaction temperature was 50 ℃, the reaction time was 10 h, Baicalein yield of 85.28%. Compared with the single-phase reaction system, the reaction rate and yield of the biphasic reaction system are improved.