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作为检测DNA单链断裂的一种新方法——碱性洗脱法,首先是由Kohn等报道,随后不少学者改进了方法,并用来进行化学致癌物引起的各种体内、外DNA损伤和修复的检测和研究。但是,利用整体动物体内处理的碱性洗脱法来快速检出环境化学致癌物,国内尚未见报道。为此,我们试用国产微孔滤膜和自己合成的3,5-二氨基苯甲酸盐化合物(简称DABA),同时用几种代表不同化学结构的间接致癌物作为方法学的验证,以图建立小鼠体
As a new method for detecting DNA single-strand breaks, the alkaline elution method was first reported by Kohn et al. Later, many scholars improved the method and used it to perform various DNA damages in vivo and in vitro caused by chemical carcinogens. Repair detection and research. However, rapid detection of environmental chemical carcinogens using the alkaline elution method of the whole animal in vivo treatment has not yet been reported in China. To this end, we tested domestically-made microporous membranes and 3,5-diaminobenzoate compounds (abbreviated as DABA) synthesized by ourselves, while using several indirect carcinogens representing different chemical structures as method validation. Establish a mouse body