论文部分内容阅读
目的:探讨mi R-101在肝细胞癌(HCC)细胞生物学行为的影响。方法:分别将mi R-101模拟物或mi RNA阴性对照序列转染Hep G2和SMMC-7721两种HCC细胞,以无处理自然生长的两种细胞为各自空白对照,观察mi R-101对两种细胞增殖、集落形成能力、凋亡及周期的影响,以及对侵袭和迁移能力的影响。结果:与各自的空白对照细胞比较,转染mi R-101模拟物后的Hep G2和SMMC-7721细胞的增值能力明显降低、细胞集落的形成明显减少、G0/G1期的细胞比例升高、细胞凋亡率明显增加、迁移与侵袭细胞数明显减少(均P<0.05);转染mi RNA阴性对照序列对两种细胞的以上指标无明显影响(均P>0.05)。结论:mi R-101可能在HCC细胞中起到了抑癌基因的作用,上调其表达能抑制HCC的恶性生物学行为。
Objective: To investigate the effect of mi R-101 on the biological behavior of hepatocellular carcinoma (HCC) cells. Methods: Two kinds of HCC cells were transfected with mi R-101 mimics or mi RNA negative control sequences respectively. Two cells without treatment of natural growth were blank control, and mi R-101 pair two The effects of cell proliferation, colony forming ability, apoptosis and cycle, and on invasion and migration ability. RESULTS: Compared with the blank control cells, the proliferation ability of Hep G2 and SMMC-7721 cells transfected with mi R-101 mimics was significantly reduced, the formation of colony was significantly reduced, the proportion of cells in G0 / G1 phase was increased, (P <0.05). The transfected miRNA negative control sequence had no significant effect on the above two indicators (all P> 0.05). Conclusion: mi R-101 may play a role of tumor suppressor gene in HCC cells. Up-regulation of mi R-101 can inhibit the malignant behavior of HCC.