以花后15d的大豆籽粒作为对照,通过Solexa高通量测序方法对花后35d、55d和65d的大豆籽粒进行转录水平上的检测,并结合GO功能注释和pathway分析,共有差异表达unigene为9 905个,其中调控脂肪酸合成途径的显著差异表达基因12个,蛋氨酸代谢途径关键基因6个。以花后55d的cDNA为样本,荧光定量PCR检测了12个差异表达基因,其结果可与测序结果互为印证。 Soybean grains at 15 days after anthesis were used as control. Solexa high-throughput sequencing was used to detect the transcriptional levels of soybean grains at 35 days, 55 days and 65 days after anthesis. Combined with GO functional annotation and pathway analysis, the common expression of unigene was 9 905, including 12 differentially expressed genes regulating the fatty acid synthesis pathway and 6 methionine metabolic pathway genes. Fifty-five days after anthesis, 12 differentially expressed genes were detected by fluorescence quantitative PCR, and the results could be confirmed with the sequencing results.