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目的:探讨Kir6.2基因E23K位点多态性与耐力训练所致的血糖、胰岛素和VO2max改变间的关系。方法:采用PCR-RFLP技术对214例美国各种族50至75岁男女受试者的Kir6.2位点E23K多态性进行基因型分析。受试者在专人监控下进行24周的耐力训练后,观察其E23K与血糖、胰岛素和VO2max之间的关系。结果:(1)三种基因型受试者的空腹血糖、胰岛素和VO2max的基础值无显著差异;(2)24周耐力训练后,三种基因型受试者的VO2max均显著升高(P<0.05),体重和体脂则显著下降(P<0.05),身高体重指数(BMI)和呼吸商(RER)则在男性各基因型中显著下降(P<0.05);(3)24周耐力训练后,三种基因型受试者的空腹胰岛素水平和曲线下面积(AUC)均显著下降(P<0.05),但训练并未引起空腹血糖及其AUC显著变化。(4)男性EK基因型RER显著低于EE和KK基因型,女性则表现为KK基因型显著高于EE基因型。结论:(1)无论E23K位点的基因型为EE、EK或KK,24周的耐力训练均可提高受试者的VO2max并降低体重、体脂、空腹胰岛素水平和AUC;(2)耐力训练对男性BMI和RER降低的影响更显著;(3)E23K多态性与RER的关系较VO2max和血糖水平更密切。
Objective: To investigate the relationship between the E23K polymorphism of Kir6.2 gene and the changes of blood glucose, insulin and VO2max induced by endurance training. Methods: PCR-RFLP was used to genotype the E23K polymorphism at Kir6.2 locus in 214 male and female subjects from 50 to 75 years of age in the United States. Subjects underwent 24-week endurance training under personal monitoring to observe their E23K and blood glucose, insulin and VO2max relationship between. Results: (1) There was no significant difference in fasting blood glucose, insulin and VO2max among three genotypes subjects. (2) After 24 weeks of endurance training, VO2max in all three genotypes significantly increased (P (P <0.05), body weight and body fat decreased significantly (P <0.05), and body mass index (BMI) and respiratory quotient (RER) After training, fasting insulin and area under the curve (AUC) were significantly decreased in all three genotype subjects (P <0.05), but training did not cause any significant changes in fasting plasma glucose and AUC. (4) The RER of male EK genotypes was significantly lower than that of EE and KK genotypes, while the female genotypes of KK genotypes were significantly higher than EE genotypes. Conclusion: (1) 24-week endurance training increased VO2max and decreased body weight, body fat, fasting insulin and AUC, regardless of E23K genotype EE, EK or KK; (2) Endurance training (3) The relationship between E23K polymorphism and RER was closer than VO2max and blood glucose level.