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目的:调查鲁西南汉族人群中正常人和强直性脊柱炎(AS)患者的HLA-B*27基因亚型的频率分布状况,并分析HLA-B*27亚型与AS的相关性。方法:对826名正常人(对照组)、76例AS患者(AS组)采用特异性引物聚合酶链反应技术(PCR-SSP)进行HLA-B*27基因及亚型检测。结果:在正常组和AS组中,共检出了11种HLA-B*27亚型,2组均以B*2704和B*2705亚型为优势亚型,2亚型分别为38.8%和41.8%。2组间的B*2704和B*2705亚型的构成比差异无统计学意义。结论:HLA-B*2704和HLA-B*2705是鲁西南地区汉族人群AS的易感基因,与鲁西南地区汉族人群AS的发病呈强相关。
Objective: To investigate the frequency distribution of HLA-B * 27 gene subtypes in normal and ankylosing spondylitis (AS) patients in the southwestern Shandong and analyze the correlation between HLA-B * 27 subtypes and AS. Methods: HLA-B * 27 gene and its subtypes were detected by PCR-SSP in 826 normal subjects (control group) and 76 AS patients (AS group). RESULTS: Eleven HLA-B * 27 subtypes were detected in the normal and AS groups. The two subtypes included B * 2704 and B * 2705 subtypes as predominant subtypes, 2 subtypes 38.8% and 41.8%. There were no significant differences in the constituent ratios of B * 2704 and B * 2705 subtypes between the 2 groups. Conclusion: HLA-B * 2704 and HLA-B * 2705 are susceptibility genes of AS in Han population of Shandong province, which are strongly correlated with the incidence of AS in Han population of Shandong province.