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目的 探索卵巢癌粘附分子CD44的变构体CD44v6表达和p5 3基因突变与卵巢癌转移之间的关系和作用机理。方法 选择正常卵巢 2 0例、良性卵巢肿瘤 2 0例、卵巢癌 45例 (其中 2 0例肿瘤无转移 ,2 5例肿瘤有转移 ) ,应用流式细胞仪测定卵巢癌细胞DNA含量及其在细胞周期各相中的分布 ;应用逆转录 聚合酶链反应 (RT PCR)及特异探针D3 对卵巢癌细胞进行DNA印渍杂交分析 ,并对杂交条带进行辉度扫描 ;应用银染聚合酶链反应 单链构象多态性 (PCR SSCP)技术检测卵巢癌细胞p5 3基因突变 ,并与正常卵巢和良性卵巢肿瘤进行比较。结果 正常卵巢、良性卵巢肿瘤、卵巢癌无转移和有转移者CD44v6表达阳性率分别为 0 %、10 %、75 %、88% ;而p5 3基因突变率分别为 0 %、5 %、40 %、6 0 % ,两者都呈逐渐上升趋势。CD44v6辉度扫描 ,卵巢癌无转移者 (382 0± 2 89)与有转移者(10 132± 15 2 1)比较 ,差异有极显著性 (P <0 .0 1)。卵巢癌CD44v6表达阳性和阴性患者中 ,G2 M期细胞平均值分别为 5 .90 %和 5 .0 6 % (P >0 .0 5 ) ;异倍体细胞分别为 5 7%和 5 0 % (P >0 .0 5 )。而在p5 3基因有突变和无突变患者中 ,G2 M期细胞分别为 11.15 %和 5 .85 % (P <0 .0 5 ) ;异倍体细胞分别为 74%和 36 % (P <0 .0 5 )。结论 CD44v6表达与
Objective To explore the relationship between CD44v6 expression in ovarian cancer adhesion molecule CD44 and the mutation of p5 3 gene and the metastasis of ovarian cancer. Methods 20 cases of normal ovary, 20 cases of benign ovarian tumors, 45 cases of ovarian cancer (20 cases of tumor metastasis, 25 cases of tumor metastasis), the determination of DNA content of ovarian cancer cells by flow cytometry and its Cell cycle phase distribution; reverse transcription polymerase chain reaction (RT PCR) and specific probes D3 ovarian cancer cells were analyzed by DNA blot hybridization, and the hybridization bands were scanned by intensity; silver staining of the polymerase Single strand conformation polymorphism (PCR SSCP) was used to detect the mutation of p5 3 in ovarian cancer cells and compared with normal ovarian and benign ovarian tumors. Results The positive rates of CD44v6 expression in normal ovary, benign ovarian tumor and non-metastatic ovarian cancer were 0%, 10%, 75% and 88%, respectively. The mutation rate of p5 3 gene was 0%, 5%, 40% , 60%, both showed a gradual upward trend. CD44v6 intensity scan, ovarian cancer without metastasis (382 0 ± 2 89) and metastasis (10 132 ± 15 2 1), the difference was significant (P <0.01). In patients with positive or negative CD44v6 expression, the average number of G2 M phase cells was 5.90% and 5.06% (P> 0.05), respectively. The number of aneuploid cells was 57% and 50% (P> 0.05). However, the number of G2 M phase cells was 11.15% and 5.85% (P <0.05) in the patients with mutation and non-mutation of p5 3 gene respectively; the aneuploid cells were 74% and 36% respectively (P <0 .0 5). Conclusion CD44v6 expression and