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目的建立稳定、可行的脑局灶性梗死动物模型,并通过功能性磁共振成像和病理学方法对其进行评价。方法采用S-D大鼠25只,随机分5组。A组为假手术对照组,B、C、D、E组大鼠分别用自创改良线栓法栓塞大鼠一侧大脑中动脉后3、6、12、24 h进行磁共振成像和波谱分析,检查结束后各组取2~3只进行病理观察,另2只TTC染色后行梗死灶形态学观察。结果对照组(A组)MR I、1H MRS及病理检查均未见异常。实验组DW I成像病灶呈高信号,1H MRS示B、C、D、E各组均见病灶NAA峰下降,Lac峰在各组均见升高,B、C、D组Cho、Cr峰未见明显异常,E组Cho、Cr峰略下降。结论该方法制作急性脑梗死实验模型是稳定的、可重复的,并且适合MRS实验研究。
Objective To establish a stable and feasible animal model of focal cerebral infarction and to evaluate it by functional magnetic resonance imaging and pathological methods. Methods 25 S-D rats were randomly divided into 5 groups. Group A was sham-operation control group, and rats in groups B, C, D and E were subjected to magnetic resonance imaging and spectral analysis at 3, 6, 12 and 24 h after embolization of rat’s middle cerebral artery At the end of the test, 2 to 3 of each group were taken for pathological observation. The other 2 TTCs were stained to observe the morphology of infarction. Results There were no abnormalities in MR I, 1H MRS and pathological examination in control group (group A). In the experimental group, DWI imaging showed high signal intensity. 1H MRS showed that the NAA peak decreased in all the groups of B, C, D and E, and Lac peak increased in all groups, Cho, Cr peak in B, C and D groups See obvious abnormalities, E group Cho, Cr peak decreased slightly. Conclusion The experimental model of acute cerebral infarction by this method is stable and reproducible, and suitable for MRS experimental study.