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目的 :建立一种简便、快速、具有特异性检测和识别变形链球菌的方法。方法 :根据葡聚糖 (dexA)基因设计的具有特异性针对变形链球菌的寡核苷酸引物 ,通过聚合酶链反应扩增一个 1 88bp的DNA片段以检测变链菌株和从人的口腔提取的菌斑和唾液标本。结果 :这种聚合酶链反应的方法检测变链菌具有特异性和敏感性。结论 :葡聚糖酶聚合酶链反应适合于特异性的检测和识别变形链球菌。
Objective: To establish a simple, rapid and specific method for the detection and identification of Streptococcus mutans. Methods: According to the dextran (dexA) gene designed oligonucleotide primers specific for Streptococcus mutans, a 188 bp DNA fragment was amplified by polymerase chain reaction to detect Streptococcus mutans and extracted from the human oral cavity Of plaque and saliva specimens. Results: This polymerase chain reaction method for detecting Streptococcus mutans was specific and sensitive. Conclusion: The glucanase polymerase chain reaction is suitable for the specific detection and identification of Streptococcus mutans.