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目的 观察低浓度镉 (39μmol L)处理长江华溪蟹 (Sinopotamonyangtsekiense)后 ,其肝胰腺细胞超微结构的变化。 方法 实验组 30只雄蟹培养在浓度为 39μmol L的氯化镉 (CdCl2 )溶液中 ;对照组 30只雄蟹培养在已放置 2d的自来水中 ,分别在镉处理后 5d、15d、30d三个不同时间段活体解剖雄蟹 ,电镜下观察肝胰腺细胞超微结构。 结果 镉对肝胰腺细胞内的主要细胞器影响表现为 :核膜从弥散状到最后解体 ;线粒体膜通透性改变 ,肿胀变形 ,嵴减少或消失 ,最后完全空泡化 ;粗面内质网先裂解为大小不同的小泡 ,尔后小泡上的核糖体开始脱落 ,直至变成同心圆状板层结构 ;出现大量滑面内质网 ;溶酶体数量随镉处理时间的延长而增多 ,形成空泡、自噬体或髓样体 ;微绒毛出现脱落和部分空泡化。 结论 镉使长江华溪蟹肝胰腺细胞的解毒功能丧失 ,破坏了肝胰腺细胞的细胞核、线粒体、粗面内质网等主要细胞器的结构与功能的完整性 ,影响了肝胰腺细胞的正常生理机能。
Objective To observe the changes of the ultrastructure of hepatopancreas cells after treatment of Sinopotamonyangtsekiense with low concentration cadmium (39μmol L). Methods Thirty male crabs were cultured in a concentration of 39μmol L cadmium chloride (CdCl2) solution. In the control group, 30 male crabs were cultured in tap water for 2 days and were exposed to cadmium for 5 days, 15 days and 30 days respectively. The section of live male anatomy, electron microscopy under the ultrastructure of hepatopancreas cells. Results The main organelles of hepatopancreas cells were as follows: the nuclear membrane diffused to the final disintegration; mitochondrial membrane permeability changed, swelling and deformation, crest decreased or disappeared, finally completely vacuolization; rough endoplasmic reticulum And then the ribosomes on the vesicles began to fall off until they became concentric lamellar structures; a large amount of synovial endoplasmic reticulum appeared; the amount of lysosomes increased with the prolongation of cadmium treatment time to form Vacuoles, autophagosomes or myeloid; microvilli shedding and partial vacuolation. Conclusion Cadmium can detoxify HepG pancreas cells of Huaxia crab in the Yangtze River and destroy the integrity of the structure and function of the major organelles such as nucleus, mitochondria and rough endoplasmic reticulum of hepatopancreas, affecting the normal physiological function of hepatopancreas cells .