目的研究牛蒡总多糖提取的最佳工艺条件。方法采用蒽酮-硫酸法测定总多糖的含量,以提取温度、提取时间、提取次数、溶剂用量为4个因素,分别设计3个水平进行正交实验。结果最佳工艺为15倍量水,95℃提取3h,共提取4次。结论提取条件对牛蒡多糖的得率具有影响。 Objective To study the optimum conditions for the extraction of total polysaccharide from Burdock. Methods The contents of total polysaccharides were determined by anthrone-sulfuric acid method. The extraction temperature, extraction time, number of extractions and solvent dosage were four factors. Three orthogonal experiments were designed. Results The best process was 15 times water, extracted at 95 °C for 3 hours and extracted 4 times. Conclusion The extraction conditions have an effect on the yield of burdock polysaccharide.