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目的 探讨TPA对人喉鳞癌细胞的抗增殖作用。方法 应用MTT、流式细胞 (FCM)技术对Hep - 2细胞进行观察和检测。结果 10nMTPA作用 12h ,可明显抑制Hep - 2细胞的增殖 ;下调PCNA的表达量 ;细胞分裂阻滞于G1期。结论 TPA能够抑制人喉鳞癌细胞的增殖 ,这种作用与细胞核PCNA的变化有密切的关系。
Objective To investigate the antiproliferative effect of TPA on human laryngeal squamous cell carcinoma. Methods Hep - 2 cells were observed and detected by MTT and flow cytometry (FCM). Results After treated with 10nMTPA for 12h, the proliferation of Hep - 2 cells was significantly inhibited. The expression of PCNA was down - regulated and the cell division was arrested in G1 phase. Conclusion TPA can inhibit the proliferation of human laryngeal squamous cell carcinoma cells. This effect is closely related to the changes of nuclear PCNA.