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目的:研究氯麻滴鼻剂含量测定方法。方法:色谱柱为SPOLAR C_(18)柱(250 mm’4.6 mm,5(m);流动相为0.02 mol·L~(-1)磷酸二氢钾溶液-甲醇(67∶33)(磷酸调p H至3.5);检测波长为220 nm。结果:氯霉素在10.43~156.42μg·ml~(-1)浓度范围内线性良好(r=0.999 9),平均回收率为99.0%,RSD为0.4%(n=9);盐酸麻黄碱在41.10~616.56μg·ml~(-1)浓度范围内线性良好(r=0.999 7),平均回收率为98.6%,RSD为0.4%(n=9)。结论:该方法操作简便,准确可靠,可作为氯麻滴鼻剂的含量测定方法。
Objective: To study the method of determination of chlorine and nasal drops. METHODS: The chromatographic column was SPOLAR C 18 column (250 mm’4.6 mm, 5 (m)). The mobile phase consisted of 0.02 mol·L -1 potassium dihydrogen phosphate solution-methanol (67:33) The detection wavelength was 220 nm.RESULTS: Chloramphenicol had a good linearity (r = 0.999 9) at a concentration range of 10.43-156.42 μg · ml -1 with an average recovery of 99.0% with a RSD of (N = 9). The average recovery was 98.6% and the RSD was 0.4% (n = 9) in the range of 41.10 ~ 616.56μg · ml ~ (-1) ) Conclusion: The method is simple, accurate and reliable, and can be used as a method for determination of chlorine and nasal drops.