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研究对象为南加里曼丹和西弗洛雷斯两个岛的成年居民。前者是疟疾中等流行区,但居民中未发现巨脾者;丝虫以亚周期型马来丝虫为主。后者是班氏与帝汉丝虫流行区,患热带巨脾综合征(TSS)的病人较多。试验中,急性期的丝虫病或疟疾病人及慢性阻塞性丝虫病人(象皮肿或鞘膜积液)均不纳入统计,因为这些病人T细胞亚群在比例上已发生明显改变。作者采用聚蔗糖-泛影酸盐梯度离心,从肝素抗凝血中分离单核细胞。并用体外白细胞总数及分类计数算出每立方毫米血中淋
The object of study is adult inhabitants of the islands of South Kalimantan and West Flores. The former is a malaria-endemic area, but residents are not found spleen; filariasis sub-type Malay worm-based. The latter is the endemic line of Banjul and Timothy filariasis, suffering from tropical splenomegaly syndrome (TSS) patients more. In the trial, neither filariasis nor malaria patients and patients with chronic obstructive filariasis (elephantiasis or hydrocele) in the acute phase were included in the statistics, as the proportion of T-cell subpopulations in these patients had changed significantly. The authors isolated monocytes from heparin anticoagulant using Ficoll-UDH gradient centrifugation. And with the total number of leukocytes in vitro and count the number of counts per cubic millimeter of blood in the shower