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目的探察证实3-NP对实验大鼠纹状体的损害作用。方法实验借助行为学探测、经典组化和免疫组化染色技术对大鼠运动行为学和纹状体组织病理学变化进行形态学探察,实验数据采用SPSS软件统计学处理。结果①运动行为学探测显示大鼠过平衡木的启动延搁时间和完成全过程时间明显延长(P<0.05,P<0.05),以及前爪滑落次数与3-NP处理之前比较具有统计学差异(P<0.05),其抓握时间也明显比3-NP处理之前延长(P<0.05)。②组织学探察显示,多种组化染色均在实验大鼠纹状体的背外侧区恒定出现一块明显的坏死灶,发生率为80%。坏死区及其周边区绝大部分神经元发生丢失,仅有少数体积较大的细胞幸存。Golgi染色显示在周边区幸存神经元的树突发生明显断裂和弯曲。酶组化显示病灶区SDH明显淡染,周边区可见一些强反应的细胞。③神经元特异性抗体探察显示坏死灶及其周边区神经元丢失严重,纹状体投射神经元的丢失更显著。对神经元死亡和损伤性质的进一步鉴定结果显示在坏死灶及其周边区均可见大量凋亡细胞。结论 3-NP特异性地诱导纹状体背外侧区神经元损伤,纹状体不同类型神经元对3-NP损伤显示不同的敏感性,凋亡和能量代谢障碍因素牵涉到此病理过程。
Objective To investigate the role of 3-NP in the striatum of experimental rats. Methods Experiments were performed to explore the morphological changes of motor and striatum histopathological changes by means of behavioral tests, classical histochemistry and immunohistochemical staining. The experimental data were processed by SPSS software. Results ① The kinetic behavior test showed that the starting delay time and the completion time of the over-balanced wood in rats were significantly prolonged (P <0.05, P <0.05), and the number of forelimb slipping was significantly different from that before 3-NP treatment <0.05). The grasping time was also significantly longer than that before 3-NP treatment (P <0.05). ② Histological examination showed that a variety of histochemical staining in the dorsal lateral striatum of experimental rat constant a clear necrosis, the incidence was 80%. Most of the neurons in the necrotic area and its peripheral area are lost, and only a few of the larger cells survive. Golgi staining revealed significant degeneration and flexing of dendrites in surviving neurons in the peripheral area. Enzymatic histochemistry showed that the SDH of the lesion area was significantly lightly stained and some strongly reactive cells were visible in the peripheral area. (3) The detection of neuron-specific antibodies showed that the neurons in the necrotic foci and their peripheral areas were severely lost, and the loss of neurons in the striatum was more pronounced. Further identification of the neuronal death and injury properties revealed that a large number of apoptotic cells were visible in both the necrotic and surrounding areas. CONCLUSION: 3-NP specifically induces neuronal damage in the dorsolateral striatum. Different neurons in striatum show different sensitivity to 3-NP injury. Apoptosis and energy metabolism disorders are involved in this pathological process.