论文部分内容阅读
目的探讨mi R-1297在他莫昔芬(TAM)介导的子宫内膜癌中的表达及临床意义。方法收集2012年3月1日至2015年3月1日期间首都医科大学附属北京天坛医院乳癌术后服用TAM超过5年的可疑内膜病变患者10例,对照组选用因子宫脱垂而行子宫切除的患者,没有激素和TAM服药史,HE染色确定病理类型。离体培养子宫内膜原代细胞,用TAM处理后采用细胞克隆方法检测细胞的生长情况,及Real time-PCR方法检测组织及细胞中mi R-1297的表达。结果对照组、服用TAM的非典型增生组、子宫内膜癌组中mi R-1297表达呈递增趋势。此外,离体培养的子宫内膜细胞,用TAM处理后发现细胞增殖,检测mi R-1297表达上调。结论在TAM介导的子宫内膜癌变中mi R-1297促进细胞生长,具有致癌作用。这能让我们更好地理解TAM介导子宫内膜癌的机制。对TAM介导的子宫内膜癌而言,mi R-1297可能是一个潜在的早期临床预测的生物标志物。
Objective To investigate the expression and clinical significance of mi R-1297 in tamoxifen (TAM) -mediated endometrial carcinoma. Methods Ten cases of suspicious endometrial lesions with TAM over 5 years after breast cancer surgery were collected from Beijing Tiantan Hospital, Capital Medical University, Beijing from March 1, 2012 to March 1, 2015. The control group was treated with uterine prolapse Excised patients, without hormone and TAM medication history, HE staining to determine the pathological type. The primary cells of endometrium were cultured in vitro. Cell growth was detected by cell cloning method after TAM treatment. The expression of mi R-1297 in tissues and cells was detected by Real time-PCR. Results The expression of mi R-1297 in the control group, the atypical hyperplasia group treated with TAM, and the endometrial carcinoma group showed an increasing trend. In addition, ex vivo cultured endometrial cells were treated with TAM and found that the proliferation of cells, the detection of mi R-1297 upregulation. Conclusions mi R-1297 promotes cell growth in TAM-mediated endometrial carcinogenesis and is carcinogenic. This allows us to better understand the mechanism of TAM-mediated endometrial cancer. For TAM-mediated endometrial cancer, mi R-1297 may be a potential biomarker for early clinical prediction.