利用响应面法,以黄酮吸附率为响应值,选取上样浓度、上样流速及上样pH值为影响因素,研究各因素及交互作用对黄酮纯化工艺的影响,对大兴安岭金莲花总黄酮大孔树脂纯化工艺进行优化。结果表明:筛选出D-101大孔树脂为较优树脂,上样浓度2.7 mg/mL、上样流速2.5 BV/h、上样pH为5。最高吸附率为71.34%,金莲花总黄酮纯度达93.41%。 Using response surface method, taking flavone adsorption rate as response value, selecting sample concentration, sample flow rate and sample pH as influencing factors, the effects of various factors and interaction on flavonoid purification process were studied. Hole resin purification process optimization. The results showed that D-101 macroporous resin was screened out as the best resin with a loading concentration of 2.7 mg / mL, a loading flow rate of 2.5 BV / h and a loading pH of 5. The highest adsorption rate was 71.34%. The purity of total flavonoids of Trollius chinensis reached 93.41%.