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目的 :了解长白山区莱姆病传播媒介 -全沟硬蜱的地理位置、分布及其病原携带情况。方法 :用酶联免疫吸附试验、间接免疫荧光试验检测莱姆病特异IgG抗体。 结果 :长白山区抓捕的 6 2 0只硬蜱 ,全沟硬蜱为5 93只 ,占总数的 95 .80 % ,用BSK培养基 33℃培养 ,分纯后共获得纯阳性伯氏疏螺旋体培养物 2 43株 ,用兔抗美国B31特异性多克隆抗体和针对莱姆病螺旋体种属特异单克隆抗体 ,间接免疫荧光抗体试验进行鉴定 ,证明其单克隆抗体反应与美国菌株稍有差异 ,与国内分离株M7相同。血检 82 6人 ,IgG、IgM≥ 1∶12 8(抗体滴度 )有 49例 ,阳性率为 5 .93% ;IgG、IgM≥ 1∶6 4(抗体滴度 )有 82例 ;二项合计为15 .85 %。结论 :长白山区是莱姆病重要疫源地 ,人群中有莱姆病感染存在 ,并有莱姆病发生
OBJECTIVE: To understand the geographic location, distribution and pathogens of Ixodes melanogaster in Lyme disease in Changbai Mountains. Methods: Lyme disease-specific IgG antibody was detected by enzyme-linked immunosorbent assay and indirect immunofluorescence assay. Results: Six hundred and twenty soft-shelled ticks were collected in Changbai Mountain area, and there were 5 93 ticks, accounting for 95.80% of the total ticks, cultured in BSK medium at 33 ℃. Pure-positive Borrelia burgdorferi A total of 2 43 cultures were identified by rabbit anti-B31-specific polyclonal antibody and species-specific monoclonal antibodies against Borrelia burgdorferi and indirect immunofluorescent antibody test. The results showed that there was a slight difference between the monoclonal antibody and the American strain, The same with the domestic isolates M7. There were 49 blood samples with IgG and IgM≥ 1:12 8 (antibody titers), the positive rate was 5.93%. There were 82 cases with IgG and IgM≥ 1: 64 (antibody titers) Total 15.85%. Conclusion: Changbai Mountain is an important source of Lyme disease. There are Lyme disease in the population and Lyme disease