论文部分内容阅读
目的观察血管局部联合转染c-myc反义寡核苷酸(AODN)和组织纤溶酶原激活物(tPA)基因对损伤后动脉内膜增生的影响。方法将同一只兔的左、右髂外动脉(各1·0cm)对换移植,移植血管分别用脂质体、c-myc-AODN和pBudCE4·1/tPA液浸泡,血管吻合口用上述3种液体浸泡过的缝线吻合。实验终点(术后3、7、14、28、56d)分为5个亚组,术后各实验终点取移植血管标本用于病理学检测、发色底物法检测tPA活性实验、3H-TdR掺入实验和免疫组织化学染色检测。结果术后各时间点联合转染组血管的内膜面积、管腔狭窄程度、3H-TdR掺入量和增值细胞核抗原(PCNA)阳性细胞数均显著低于对照组(P<0·01),而且亦明显低于c-myc-AODN和tPA单独转染组(P(0·05)。结论血管局部联合转染c-myc-AODN和tPA基因能有效抑制内膜增生,防止损伤血管狭窄。
Objective To observe the effects of vascular local c-myc antisense oligonucleotide (AODN) and tissue plasminogen activator (tPA) on intimal hyperplasia after injury. Methods The left and right external iliac arteries (1.0 cm each) were transplanted in the same rabbit. The implanted vessels were soaked in liposome, c-myc-AODN and pBudCE4.1 / tPA respectively. The kind of liquid soaked suture line. At the end of the experiment (3, 7, 14, 28 and 56 days after operation), the rats were divided into 5 subgroups. The end of each experiment was used to test the pathology of the transplanted blood vessels. The tPA activity was detected by the chromogenic substrate method. 3H- Incorporation test and immunohistochemical staining. Results The intima area, the degree of stenosis, the amount of 3H-TdR incorporation and the number of proliferating cell nuclear antigen (PCNA) positive cells in the transfection group were significantly lower than those in the control group at each time point after operation (P <0.01) , But also significantly lower than that of c-myc-AODN and tPA alone transfected group (P <0.05) .Conclusion Vascular local c-myc-AODN and tPA transfection can effectively inhibit intimal hyperplasia and prevent vascular stenosis .