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目的探讨慢性乙型肝炎T细胞亚群与HBV DNA的关系。方法用流式细胞仪直接免疫荧光法测算318例慢性乙型肝炎及22例正常对照者的外周血T细胞亚群(CD3~+、CD4~+、CD8~+)百分率,用荧光定量PCR法检测慢性乙型肝炎HBV DNA,比较不同HBV DNA载量慢性乙型肝炎外周血T细胞亚群的变化。结果慢性乙型肝炎外周血CD_3~+、CD_4~+、CD_8~+、CD_4~+/CD_8~+比正常对照组降低(P<0.05)。HBV DNA高载量的慢性乙型肝炎者CD_4~+ T细胞百分率及CD_4~+/CD_8~+较低载量者减少(P<0.05),CD_8~+T细胞百分率增加(P<0.05)。结论 HBVDNA复制增加可加重慢性乙型肝炎T细胞亚群的紊乱,CD_4~+/CD_8~+的动态变化可提示HBV感染者细胞免疫功能的变化。
Objective To investigate the relationship between T cell subsets and HBV DNA in chronic hepatitis B patients. Methods The percentage of T lymphocyte subsets (CD3 ~ +, CD4 ~ +, CD8 ~ +) in 318 patients with chronic hepatitis B and 22 healthy controls were measured by flow cytometry direct immunofluorescence. The HBV DNA levels of chronic hepatitis B were detected and the changes of T cell subsets in peripheral blood of patients with chronic hepatitis B were compared. Results The levels of CD_3 ~ +, CD_4 ~ +, CD_8 ~ + and CD_4 ~ + / CD_8 ~ + in the peripheral blood of patients with chronic hepatitis B were lower than those in the control group (P <0.05). The percentage of CD_4 ~ + T cells and CD_4 ~ + / CD_8 ~ + with low HBV DNA load were lower (P <0.05) and the percentage of CD_8 ~ + T cells were increased in chronic hepatitis B patients with high HBV DNA load (P <0.05). Conclusion The increase of HBVDNA replication may aggravate the disorder of T cell subsets in chronic hepatitis B. The dynamic changes of CD_4 ~ + / CD_8 ~ + may indicate the changes of cellular immune function in HBV infected patients.