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目的:探讨益肺宣肺降浊方对血管性痴呆大鼠海马神经细胞凋亡和关键因子c AMP、PKA表达的影响以及其部分作用机制。方法:80只健康SD大鼠随机分为假手术组、模型组、治疗组和对照组,每组20只。假手术组、模型组予盐水灌胃;治疗组予益肺宣肺降浊方,对照组予吡拉西坦。4组均采用Morris水迷宫实验检测大鼠记忆与空间探索能力,采用放射免疫法检测海马c AMP含量,westernblot法检测海马PKA表达。结果:与模型组比较,假手术组、治疗组及对照组逃避潜伏期缩短、跨越平台次数增多、目标象限停留时间延长(P<0.05);c AMP含量和PKA表达增高(P<0.05);与对照组比较差异无统计学意义(P>0.05)。结论:益肺宣肺降浊方可明显提高血管性痴呆大鼠学习记忆功能和空间探索能力,减少神经细胞凋亡及提高海马c AMP、PKA的表达水平,其作用机制之一可能是通过影响c AMP/PKA信号转导通路关键因子c AMP、PKA的表达来实现。
Objective: To investigate the effects of Yifei Xuanfei Jiangzhuo Fang on the apoptosis of hippocampal neurons and the expression of c AMP and PKA in hippocampus of rats with vascular dementia and their partial mechanisms. Methods: Eighty healthy SD rats were randomly divided into sham operation group, model group, treatment group and control group, with 20 rats in each group. Sham-operated group and model group were treated with saline. The treatment group was given Xifei Jiangzhuo Decoction, while the control group was treated with Piracetam. Morris water maze test was used to detect memory and space exploration ability of rats in each group. The content of cAMP in hippocampus was detected by radioimmunoassay and PKA expression in hippocampus was detected by western blot. Results: Compared with the model group, the escape latency, the number of crossing platforms and the target quadrant prolonged (P <0.05), the cAMP content and PKA expression increased (P <0.05) There was no significant difference in the control group (P> 0.05). Conclusion: Yifei Xuanfei Jiangzhuo can significantly improve the ability of learning and memory and space exploration of vascular dementia rats, reduce the apoptosis of neurons and increase the expression of cAMP and PKA in the hippocampus. One of the mechanisms may be through the influence of c AMP / PKA signal transduction pathway key factor c AMP, PKA expression to achieve.