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目的 了解He Ne激光照射尖锐湿疣 (CA)组织后对人乳头瘤病毒 (humanpapillomavirus,HPV)载量的影响。方法 用荧光定量PCR方法检测CA组织中HPV DNA拷贝数 ,比较CO2 激光治疗CA 4周后 ,He Ne激光组与对照组HPV DNA下降平均值及各组在治疗观察前、后的HPV DNA变化情况。结果CO2 激光治疗观察 4周后 ,HPV DNA的平均下降值He Ne激光组 :(4.0 4± 1 .90 )× 1 0 5copies/mg ,对照组 :(- 1 .1 5± 8.78)× 1 0 4 copies/mg,两组比较t =1 0 .90 ,P =0 .0 0 0 1 ;治疗观察前、后HPV DNA量变化的自身对照 ,He Ne激光组 :t=1 1 .68,P =0 .0 0 0 1 ;对照组 :t=0 .72 ,P =0 .4789。He Ne激光组与对照组在治疗观察后 2周和 4周CA复发率分别为 :2 3 .33 % ,40 .0 0 % ;30 .0 0 % ,60 .0 0 %。结论 低能量的He Ne激光照射CA组织可降低其HPV载量 ,并可减少CA在CO2 激光治疗后 4周的复发率
Objective To investigate the effect of He Ne laser irradiation on human papillomavirus (HPV) load. Methods The HPV DNA copy number in CA tissue was detected by real-time fluorescence quantitative PCR. The changes of HPV DNA in He Ne laser group and control group after 4 weeks of CO2 laser treatment were compared with those in control group. The changes of HPV DNA in each group before and after treatment . Results After 4 weeks of CO2 laser treatment, the average decrease of HPV DNA in HeNe laser group was (4.04 ± 1.90) × 105copies / mg, and the control group was (-1.1 ± 8.78) × 10 4 copies / mg, the two groups compared t = 1.90, P = 0.00001; treatment before and after treatment of HPV DNA changes in the self-control, He Ne laser group: t = 1168, P = 0 .0 0 0 1; control group: t = 0 .72, P = 0 .4789. He Ne laser group and control group in the treatment of 2 weeks and 4 weeks after the CA recurrence rates were: 23.33%, 40.0%; 30.0%, 60.0%. Conclusion The low energy He Ne laser irradiation of CA tissue can reduce the HPV load and reduce the recurrence rate of CA after 4 weeks of CO2 laser treatment