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目的研究某污灌区蔬菜中有机提取物对小鼠肝、肾组织的氧化损伤作用。方法于2007年5月,选取某污灌农田出产的大白菜为研究对象,地下水灌溉农田出产的大白菜为对照,采用超声振荡法提取白菜中的有机污染物。将40只健康清洁级昆明小鼠按体重随机分为5组,分别为对照组(二甲亚砜),对照区低剂量组、对照区高剂量组、污灌区低剂量组、污灌区高剂量组,雌雄各半。高、低剂量组染毒剂量(以白菜干重计)分别为60、10g/(kg·d)。采用灌胃方式进行染毒,每日1次,灌胃容量为5ml/kg,连续染毒2周。于第15天称重、处死小鼠,测定肝、肾组织中总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)含量。结果各组小鼠体重变化差异无统计学意义(P>0.05)。与溶剂对照组比较,污灌区高、低剂量组肾脏系数和肝组织中MDA含量及高剂量组肝组织中T-SOD活力均降低,污灌区高、低剂量组肾组织中T-SOD活力、污灌区高剂量组肾组织中MDA含量均降低,差异有统计学意义(P<0.05或P<0.01)。与对照区高剂量组比较,污灌区高剂量组肾组织中MDA含量降低,差异有统计学意义(P<0.05)。而各组肝、肾组织中GSH-Px活力间比较,差异均无统计学意义(P>0.05)。结论该污灌区白菜有机提取物对小鼠肝、肾组织可产生氧化损伤作用。
Objective To study the oxidative damage effect of organic extracts from vegetables in a sewage irrigation area on the liver and kidney of mice. Methods In May 2007, Chinese cabbage produced from a sewage irrigation farmland was selected as the research object, and the Chinese cabbage produced from irrigated farmland was used as control. The organic pollutants in cabbage were extracted by ultrasonic vibration. Forty healthy Kunming mice were randomly divided into five groups according to body weight: control group (dimethyl sulfoxide), low-dose control group, high-dose control group, low-dose sewage treatment group, Group, male and female. The doses of high and low dose groups (based on the dry weight of cabbage) were 60 and 10 g / (kg · d), respectively. The method of administration by gavage once a day, gavage volume of 5ml / kg, continuous exposure to two weeks. The mice were weighed and sacrificed on the 15th day to determine the activities of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) content. Results There was no significant difference in the body weight of mice in each group (P> 0.05). Compared with the solvent control group, the renal coefficient and the content of MDA in the hepatic tissue and the T-SOD activity in the liver tissue of the high-dose and low-dose groups of sewage irrigation area decreased. The activities of T-SOD, The content of MDA in renal tissue of high-dose sewage treatment group was significantly lower than that in the high-dose sewage treatment group (P <0.05 or P <0.01). Compared with the high-dose control group, the content of MDA in the high-dose irrigation group decreased significantly (P <0.05). However, there was no significant difference in the activities of GSH-Px between the liver and kidney (P> 0.05). Conclusion The organic extract of cabbage in the sewage irrigation area can produce oxidative damage to the liver and kidney of mice.