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目的探索氢醌(hydroquinone,HQ)致DNA整体低甲基化的分子机制。方法以磷酸盐缓冲液(PBS)溶解HQ,以PBS处理组为对照组,分别以2.5、5.0、10.0和20.0μmo/lL HQ染毒TK6细胞为处理组。应用实时荧光定量-聚合酶链反应检测DNA甲基转移酶DNMT1、DNMT3a和DNMT3b的表达水平。结果与对照组相比,DNMT1、DNMT3a和DNMT3b的mRNA表达量在各HQ处理组细胞中均下降,其中以20.0μmo/lL组细胞的下降最为明显,分别下降46%(P<0.05)、83%(P<0.05)和48%(P<0.05),且DNMT1和DNMT3a的表达量随着HQ剂量的增加而下降。结论 HQ致DNA整体低甲基化下调机制可能与DNMT1、DNMT3a和DNMT3b表达异常有关。
Objective To explore the molecular mechanism of global hypomethylation of DNA induced by hydroquinone (HQ). Methods HQ was dissolved in phosphate buffered saline (PBS) and treated with PBS as the control group. TK6 cells were treated with 2.5, 5.0, 10.0 and 20.0 μmo / L HQ, respectively. The expression levels of DNA methyltransferase DNMT1, DNMT3a and DNMT3b were detected by real-time fluorescence quantitative polymerase chain reaction. Results Compared with the control group, the mRNA expression of DNMT1, DNMT3a and DNMT3b decreased in all HQ treated groups, with the most significant decrease being 20.0μmo / L in the group of cells decreased 46% (P <0.05), 83 % (P <0.05) and 48% (P <0.05) respectively. The expression of DNMT1 and DNMT3a decreased with the increase of HQ dose. Conclusion Down-regulation of DNA hypomethylation in HQ may be related to abnormal expression of DNMT1, DNMT3a and DNMT3b.