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目的:探讨山药多糖对2型糖尿病大鼠糖代谢及关键酶己糖激酶(HK)、琥珀酸脱氢酶(SDH)及苹果酸脱氢酶(MDH)活性的影响,为山药多糖对2型糖尿病治疗的研究提供依据。方法:采用高热量饮食并结合小剂量链脲佐菌素(STZ)腹腔注射的方法制备实验性2型糖尿病大鼠模型,分成正常对照组、糖尿病模型组、山药多糖治疗组(100、70、40mg/kg3个剂量组)和阳性对照组(二甲双胍,100mg/kg)共6组,治疗4周;然后检测HK、SDH和MDH活性。结果:与糖尿病模型组比较,山药多糖显示明显的降血糖作用;山药多糖中、高剂量组的2型糖尿病大鼠的HK、SDH、MDH活性与模型组比较有极显著性差异(P<0.001);山药多糖低剂量组除HK的活性与模型组比较有显著差异外(P<0.05),SDH和MDH活性都无显著差异(P>0.05)。结论:山药多糖对2型糖尿病的治疗机制之一可能是山药多糖直接或间接地提高了糖代谢或关键酶的酶活性。
Objective: To investigate the effects of yam polysaccharide on glucose metabolism and key enzymes of hexokinase (HK), succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) in type 2 diabetic rats, Diabetes treatment provides the basis for the study. Methods: Experimental type 2 diabetic rat model was established by intraperitoneal injection of high dose of streptozotocin (STZ) and high calorie diet. The rats were divided into normal control group, diabetic model group, yam polysaccharide treatment group (100, 70, 40mg / kg3 dose group) and positive control group (metformin, 100mg / kg) for 6 weeks. Then the activity of HK, SDH and MDH were detected. Results: Compared with diabetic model group, the yam polysaccharide showed obvious hypoglycemic effect. The activity of HK, SDH and MDH in type 2 diabetes mellitus rats with high and middle dosage of yam polysaccharide were significantly different from those in model group (P <0.001 (P <0.05). The activities of SDH and MDH were not significantly different (P> 0.05). Conclusion: One of the therapeutic mechanisms of yam polysaccharides on type 2 diabetes mellitus may be that Yam polysaccharide directly or indirectly enhances glucose metabolism or enzyme activity of key enzymes.