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目的研究α粒子和4-甲基亚硝基-1-(3-吡啶基)-1-丁酮(NNK)联合作用的细胞遗传毒性。方法永生化的人支气管上皮细胞(BEP2D细胞)分为正常对照组(NC)、α粒子单纯照射组(α)、NNK染毒组(NNK)、NNK染毒(100μg/ml)后α粒子照射组(NNK+α)和α粒子照射后NNK染毒(100μg/ml)组(α+NNK);用单细胞凝胶电泳法检测DNA损伤;用多核细胞法检测细胞次黄嘌呤鸟嘌呤核糖转移酶(HPRT)基因突变率;用细胞遗传学方法检测细胞微核率。结果与相同剂量NNK或α粒子单独作用比较,α粒子和NNK联合作用诱发BEP2D细胞DNA损伤、HPRT基因突变率、以及细胞微核率均显著增高;扣除NNK效应后,α粒子和NNK联合作用诱发BEP2D细胞DNA损伤、HPRT基因突变率、以及细胞微核率也明显高于α粒子单独照射组。结论α粒子合并NNK的细胞遗传毒性具有协同性。
Objective To study the cytogenetic cytotoxicity induced by the combination of α-particle and 4-methyl-nitroso-1- (3-pyridyl) -1-butanone (NNK) Methods Immortalized human bronchial epithelial cells (BEP2D) were divided into three groups: normal control group (NC), α-irradiation group (NNK) and NNK (100μg / ml) (NNK + α) and NNK (α + NNK) groups after exposure to α particles; DNA damage was detected by single cell gel electrophoresis; and the hypoxanthine guanine ribose sugar transfer Enzyme (HPRT) gene mutation rate; detection of cell micronucleus rate using cytogenetic method. Results Compared with the same dose of NNK or α particles, the combination of α particles and NNK induced the DNA damage, the mutation rate of HPRT gene and the rate of micronuclei in BEP2D cells. After the NNK effect was deducted, the combination of α particles and NNK induced The DNA damage of BEP2D cells, the mutation rate of HPRT gene and the rate of micronucleus were also significantly higher than those of α-particle alone. Conclusion Cytotoxicity of α particles combined with NNK is synergistic.