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目的 研究儿童系统性红斑狼疮 (SLE)与人类白细胞抗原 (HLA) A、B、DR等位基因的相关性。方法 对 5 3例SLE患儿及 78例正常对照儿童 ,应用微量淋巴毒实验和聚合酶链反应结合序列特异性多态性 (PCR SSP)分析方法分别行HLA Ⅰ类抗原及HLA Ⅱ类基因检测。结果 发现SLE患儿HLA Ⅰ类抗原A9、A11、B5、B17较正常对照儿童升高 ,与SLE发病相关。HLA Ⅱ类基因DRB1 15、DRB1 0 3基因频率较正常对照儿童明显增高 ,与SLE发病相关。而DRB1 0 4基因频率明显降低 ,为疾病保护基因。其中 ,HLA基因的型别不同 ,可影响SLE的临床表现 ,狼疮肾炎主要与HLA DRB1 0 3基因相关 ,而DRB1 15则与各型SLE相关。结论 系统性红斑狼疮患儿携带HLA A9、A11、B5、B17、HLA DRB1 15、DRB1 0 3基因与SLE易感有关。其中HLA型别不同 ,影响SLE患儿的临床表型。DRB1 0 4基因频率降低 ,为疾病保护性基因
Objective To study the association of childhood systemic lupus erythematosus (SLE) with human leukocyte antigen (HLA) A, B and DR alleles. Methods Totally 53 children with SLE and 78 normal control children were enrolled in this study. The detection of HLA class I antigens and HLA class II genes were performed by means of microlymph lymphadenosis and polymerase chain reaction-specific sequence-specific polymorphism (PCR SSP) . The results showed that the HLA class I antigens A9, A11, B5 and B17 in children with SLE were higher than those in normal controls, and were associated with the pathogenesis of SLE. HLA class II genes DRB1 15, DRB1 0 3 gene frequency was significantly higher than the normal control children, and SLE pathogenesis. The DRB1 0 4 gene frequency was significantly reduced, as the disease protection gene. Among them, different HLA genotypes may affect the clinical manifestations of SLE. Lupus nephritis is mainly associated with HLA DRB103, while DRB15 is associated with various types of SLE. Conclusion Children with systemic lupus erythematosus carrying HLA A9, A11, B5, B17, HLA DRB1 15, DRB1 0 3 gene and SLE susceptibility. Which HLA type is different, affecting the clinical phenotype of children with SLE. DRB1 0 4 gene frequency decreased, as a disease protective gene