研究金属离子与ATP的相互作用方式,对于阐明金属离子与ATP参与的酶反应过程具有重要的意义。31P NMR光谱结果表明ATP分子的α-P在pH 2—8研究范围内都不参与键合作用,而β-P和γ-P都参与键合作用。1H NMR光谱结果表明,当ATP与Mg2+发生相互作用时,其磷酸根阴离子与腺嘌呤碱基都会参与配位作用。紫外吸收光谱结果表明在碱性条件时,Mg2+会引起ATP分子发生碱基堆积,而在酸性条件时,碱基堆积则不会发生。 Studying the mode of interaction between metal ions and ATP is of significance for elucidating the enzymatic reaction involved in the interaction between metal ions and ATP. The results of 31P NMR showed that α-P of ATP molecule did not participate in the bonding in the range of pH 2-8, while both β-P and γ-P were involved in the bonding. The results of 1H NMR showed that when ATP interacted with Mg2 +, both phosphate anions and adenine bases would participate in the coordination. UV absorption spectra showed that under alkaline conditions Mg2 + caused the accumulation of ATP molecules, whereas in acidic conditions, the accumulation of bases did not occur.