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目的探讨创伤失血性休克后肠系膜淋巴液对中性粒细胞(polymorphonuclearneutrophil,PMN)的活化作用。方法检测大鼠休克后肠系膜淋巴液直接活化 PMN(CD11b、CD18的表达及过氧化物的形成)的能力。将18只健康成年雄性 SD 大鼠随机分为3组,将3组肠系膜淋巴管插管,收集淋巴液。A 组经股动脉放血,使平均动脉压降至(40±5)mm Hg,维持90 min,并按失血量三倍输注乳酸钠林格氏液;B 组收集淋巴液,但不放血,并输入与 A 组等量的乳酸林格氏液2 h;C 组只收集淋巴液,但不放血、不输液。收集放血前1 h、复苏后第1 h 及第2 h 的淋巴液。用3个时间段收集的淋巴液诱导未作过任何处理的大鼠全血,用流式细胞仪检测 PMN CD11b、CD18的表达及过氧化物的形成。所有数据以均数±标准差(x±s)表示,采用方差分析做数据处理,多组间两两均数比较采用 q 检验。结果 A 组复苏后第1h、第2 h 淋巴液诱导 PMN 后,CD11b 的表达分别为63.28%±1.13%,61.23%±1.16%,明显高于 B 组及 C 组(P<0.05);CD18的表达分别为64.02%±0.86%,62.57%±0.54%,显著高于 B 组及 C 组(P<0,05);产生过氧化物分别为66.62%±0.56%,65.47%±0.56%,显著高于 B 组及 C 组(P<0.05)。结论大鼠创伤性休克及复苏后肠系膜淋岜液能活化中性粒细胞。
Objective To investigate the effect of mesenteric lymph on the activation of polymorphonuclear neutrophil (PMN) after traumatic hemorrhagic shock. Methods The ability of direct activation of PMN (expression of CD11b, CD18 and formation of peroxide) in mesenteric lymph of rats after shock was tested. Eighteen healthy adult male Sprague-Dawley rats were randomly divided into three groups, and three groups of mesenteric lymphatic vessels were intubated to collect lymph. Group A was bled via the femoral artery and the mean arterial pressure was decreased to (40 ± 5) mm Hg for 90 min. The patient was infused with Ringer’s lactate at a rate of three times the amount of blood loss. In group B, lymphatic fluid was collected without bleeding and Enter the same amount of Group A lactate Ringer’s solution 2 h; Group C only lymphatic fluid collection, but not bleeding, do not infusion. Lymphs were collected 1 h before blood withdrawal and 1 h and 2 h after resuscitation. Whole blood of rats was induced by lymph collected in three time periods, and the expression of PMN CD11b, CD18 and the formation of peroxide were detected by flow cytometry. All data were expressed as mean ± standard deviation (x ± s), using ANOVA data processing, comparison between groups of more than two by q test. Results The expression of CD11b was 63.28% ± 1.13% and 61.23% ± 1.16% in group A at 1h and 2h after reperfusion, respectively, which were significantly higher than those in group B and C (P <0.05) The expression levels of peroxides were 64.02% ± 0.86% and 62.57% ± 0.54%, respectively, which were significantly higher than those in group B and C (P <0,05). The peroxide production was 66.62% ± 0.56% and 65.47% ± 0.56% Higher than those in group B and group C (P <0.05). Conclusions Mesenteric lymph fluid can activate neutrophils after traumatic shock and resuscitation in rats.