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目的研究慢性阻塞性肺疾病(COPD)急性加重期外周血树突细胞(DC)及自然杀伤细胞(NK)的变化。方法选择COPD患者32例,其中稳定期19例(稳定期组)、急性加重期未并发呼吸衰竭17例(加重期组)、急性加重期并发呼吸衰竭15例(呼吸衰竭组);健康查体者20例为对照组。采用免疫荧光抗体标记法及流式细胞仪检测外周血DC亚群(CD123+、CD11+)及NK(CD16+CD56+)。结果四组CD123+、CD11+、CD16+CD56+细胞比较有统计学差异(P<0.01)。加重期组及呼吸衰竭组CD123+、CD11+均低于稳定期组或对照组(P均<0.05)。稳定期组、加重期组及呼吸衰竭组CD16+CD56+低于对照组(P<0.05或<0.01),呼吸衰竭组低于稳定期组(P<0.05)。结论 COPD患者存在细胞免疫功能异常,其外周血DC、NK下降,急性加重期尤为明显;DC、NK均参与了COPD气道炎症过程。
Objective To study the changes of peripheral blood dendritic cells (DCs) and natural killer cells (NK) in patients with chronic obstructive pulmonary disease (COPD) exacerbation. Methods Thirty-two patients with COPD were selected, including 19 stable patients (stable group), 17 patients with acute exacerbation of respiratory failure (exacerbation group) and 15 patients with acute exacerbation of respiratory failure (respiratory failure group) 20 cases as control group. The peripheral blood DC subpopulations (CD123 +, CD11 +) and NK (CD16 + CD56 +) were detected by immunofluorescence antibody labeling and flow cytometry. Results The four groups of CD123 +, CD11 +, CD16 + CD56 + cells were statistically different (P <0.01). Both CD123 + and CD11 + in the exacerbation group and the respiratory failure group were lower than those in the stable group or the control group (all P <0.05). CD16 + CD56 + in stable group, exacerbation group and respiratory failure group were lower than those in control group (P <0.05 or <0.01), those in respiratory failure group were lower than those in stable group (P <0.05). CONCLUSIONS: COPD patients have abnormal cellular immune function, and the levels of DC and NK in peripheral blood are declining, especially in acute exacerbation. Both DC and NK are involved in the airway inflammation of COPD.