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提出一种腹泻性贝毒素—软海绵酸的检测方法。贝类组织经甲醇水溶液浸提后，经多氯甲烷提取，９－亚甲基重氮蒽衍生化和固相净化后，在ＨＰＬＣ的Ｃ１８柱上得以分离，并能在Ｅｘ＝２５４ｎｍ，Ｅｍ＝４１２ｎｍ的荧光检测器上灵敏的检测。该方法对样品的最低检测浓度为１ｎｇ／ｇ，衍生化及固相净化的回收率＞９５％，重复检测的相对标准差＜６％。
A method for the detection of diarrhea toxin - sponge acid was proposed. After the shellfish tissues were extracted with methanol aqueous solution, they were separated by HPLC on C18 column after derivatization with chloroform, 9-methylene diazanthrene derivatization and solid phase purification. 412nm fluorescence detector sensitive detection. The method has the lowest detection concentration of 1ng / g, the recovery of derivatization and solid-phase purification> 95%, and the relative standard deviation of repeated detection <6%.