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目的 了解狼疮样肾炎鼠白细胞介素 17(IL 17)的表达及其作用。方法 建立一种狼疮样肾炎动物模型即鼠慢性移植物抗宿主病 (cGVHD)模型 ,用放射免疫法测定尿白蛋白水平 ;用酶联免疫吸附试验 (ELISA)法测定血、尿IL 17水平 ;用免疫组织化学方法检测IL 17蛋白在肾组织的表达 ;用逆转录 聚合酶链反应 (RT PCR)分析肾组织IL 17mRNA的表达。结果 模型鼠组较对照鼠组血IL 17水平不高 ,尿IL 17水平明显升高 [(2 2± 6 7) pg/ml比 (3 1± 2 1)pg/ml];肾小球毛细血管襻、部分系膜细胞、肾小管和间质浸润的单个核细胞均有IL 17蛋白表达 ;肾组织IL 17mRNA水平明显升高 (0 34± 0 15比 0 ) ,且肾组织IL 17+ 细胞数明显增多 [Ng :(2 5± 8)cell/gcs比 0 ;Nt:(2 3± 8)cell/mm2 比 0 ];模型鼠肾组织中IL 17+ 细胞数与尿白蛋白和尿IL 17水平呈明显正相关。结论 IL 17在狼疮样肾炎鼠肾组织表达明显增加 ,并在其发病中可能起重要作用。
Objective To investigate the expression and effect of interleukin 17 (IL 17) in lupus-like nephritis rats. Methods A rat model of chronic graft-versus-host disease (cGVHD), a rat model of lupus-like nephritis, was established. Urinary albumin levels were determined by radioimmunoassay. Serum and urinary IL-17 levels were measured by enzyme linked immunosorbent assay (ELISA) The expression of IL-17 protein in renal tissues was detected by immunohistochemical method. The expression of IL-17 mRNA in renal tissues was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). Results Compared with the control group, the levels of IL-17 and IL-17 in model group were significantly higher than those in control group [(2 2 ± 6 7) pg / ml ratio (3 1 ± 2 1) pg / ml] The expression of IL-17 protein was detected in all the neovasculature, some mesangial cells, tubulointerstitial and interstitial infiltration mononuclear cells. The level of IL-17 mRNA in renal tissue was significantly increased (0 34 ± 0 15 vs 0) (2 3 ± 8) cell / mm2 than 0]. The number of IL 17+ cells in the kidney of model mice was positively correlated with urinary albumin and urine IL 17 levels were significantly positive correlation. Conclusion The expression of IL-17 in renal tissue of lupus-like nephritis mice increased significantly and may play an important role in the pathogenesis of lupus-like nephritis.