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目的 对在畜间疫病流行区 ,新发现致病性产H2 S李斯特氏菌进行生物学特性研究 ,以确定其在微生物学中地位 ,为其命名提供依据。方法 从形态染色、培养特性、生化反应、抗原性方面鉴定 ,并利用分子生物学技术作DNAG +Cmol%含量测定 ,PCR、16SrRNA序列检测同源性。结果 通过表型生物学特性鉴定 ,产H2 S李斯特氏菌与已知 7型李斯特菌不同 ,其G +Cmol%为 3 0 .5 %、16SrRNA序列分析同源性与无害李斯特菌 (L .im)为 71.0 % ,灰色李斯特菌 (L .g)为 3 7.8% ,西氏李斯特菌 (L .s) 2 8.4 %。结论 经系统发育树分析 ,证实产H2 S李斯特氏菌为李斯特氏菌属 ,国际上首次报告的新种。
OBJECTIVE To study the biological characteristics of pathogenic H2S-producing Listeria in endemic areas of epidemic disease in order to confirm its position in microbiology and provide a basis for its nomenclature. Methods Morphological staining, culture characteristics, biochemical reactions, antigenicity identification, and the use of molecular biology techniques for determination of DNAG + C mol%, PCR, 16SrRNA sequence homology. Results By phenotypic biological characterization, Listeria monocytogenes was different from the known Listeria 7 strains, the G + C mol% was 35.5%, 16S rRNA sequence analysis homology and harmless Listeria monocytogenes (L · im) was 71.0%, that of Listeria monocytogenes (L · g) was 3 7.8%, and that of Listeria monocytogenes (L · s) was 2 8.4%. Conclusion According to phylogenetic tree analysis, it is confirmed that Listeria monocytogenes is Listeria, the first reported species in the world.