HBV DNA定量能反映乙型肝炎病毒的复制情况,在临床诊断、治疗等方面具有重要的参考价值[1]。在留取标本时要求较为严格,一般规定溶血者为不合格标本,因为其中的血红蛋白(Hb)在核酸提取时可能无法彻底去除,会抑制核酸扩增从而影响结果的准确性[2]。目前,诸多试剂的检测下限为1.0 Quantitative HBV DNA can reflect the replication of hepatitis B virus, in clinical diagnosis, treatment and other aspects of an important reference value [1]. In the specimens taken more stringent requirements, the general provisions of the hemolytic were substandard specimens, because the hemoglobin (Hb) in the nucleic acid extraction may not be completely removed, will inhibit nucleic acid amplification and thus affect the accuracy of the results [2]. At present, the detection limit of many reagents is 1.0