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目的探究死亡受体途径、线粒体途径与氟诱导肾脏细胞凋亡的关系。方法48只SD大鼠随机分为4组:对照组、低氟组、中氟组和高氟组,每组12只,分别饮用含氟化钠浓度为0、50、100和200 mg/L的去离子水,染毒120天,观察氟斑牙形成情况,称体重,测尿氟含量。制备肾脏细胞悬液,流式细胞术(FCM)检测肾脏细胞凋亡。免疫组化检测肾脏组织中Caspase-3、Caspase-8、Caspase-9及细胞色素C(Cyt C)蛋白表达。结果与对照组比较,低氟组、中氟组、高氟组肾脏细胞凋亡率显著增高(P<0.05)。与对照组比较,中氟组、高氟组Caspase-8、Cyt C、Caspase-9、Caspase-3阳性表达的平均光密度值明显增高(P<0.05)。结论死亡受体途径、线粒体途径可能同时参与了氟诱导大鼠肾脏细胞凋亡的过程。
Objective To explore the relationship between death receptor pathway, mitochondrial pathway and fluorine-induced renal cell apoptosis. Methods Forty eight Sprague-Dawley rats were randomly divided into 4 groups: control group, low fluoride group, medium fluoride group and high fluoride group, with 12 rats in each group. The rats were given sodium fluoride at concentrations of 0, 50, 100 and 200 mg / L Of deionized water, 120 days of exposure to observe the formation of dental fluorosis, weighing, measuring urinary fluoride. The renal cell suspension was prepared and the apoptosis of renal cells was detected by flow cytometry (FCM). Immunohistochemistry was used to detect the expression of Caspase-3, Caspase-8, Caspase-9 and Cyt C in renal tissues. Results Compared with the control group, the apoptotic rate of renal cells was significantly increased in low fluoride group, middle fluoride group and high fluoride group (P <0.05). Compared with the control group, the mean optical density of positive expression of Caspase-8, Cyt C, Caspase-9 and Caspase-3 in middle fluoride group and high fluoride group were significantly increased (P <0.05). Conclusion The death receptor pathway and mitochondrial pathway may be involved in the process of fluoride-induced apoptosis in rat kidney.