论文部分内容阅读
用来自玉米 (ZeamaysL .)与二倍体多年生类玉米 (Z .diploperennisIltis,DoebleyandGuzm偄ｎ)杂交的孤雌生殖后代同一抗病个体的 4个株系进行了基因组原位杂交 ,用改进的杂交技术获得了近 10 0 %的检出率 ,每一检出片段在同源染色体两成员和每两个姊妹染色单体上均有清晰的信号。
Genomic in situ hybridization was performed with 4 lines of the same disease-resistant individuals from parthenogenetic offspring of Zea mays L. crossed with diploid perennial maize (Z. diploperennisIltis, Doebley and Guzmán) using improved hybridization techniques A detection rate of nearly 100% was obtained, with each detected fragment having a clear signal on both members of the homologous chromosome and on every two sister chromatid.