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目的研究无创性肢体缺血预适应对心肌缺血/再灌注损伤的保护作用,并从氧化-抗氧化角度初步探讨其作用机制。方法大鼠被随机分为缺血/再灌注(I/R)、心脏缺血预适应(CIP)和肢体缺血预适应(LIP)3组。LIP组连续3d经历左后肢缺血预适应。d4,各组动物均经历心肌缺血/再灌注损伤,CIP组于缺血前行心肌缺血预适应。连续监测心电图变化,检测心肌梗死范围,测定心肌组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、黄嘌呤氧化酶(XOD)活性以及丙二醛(MDA)含量。结果与I/R组比较,LIP及CIP组ST-段抬高幅度降低,室早出现时间推迟,持续时间缩短,室性心律失常发生率降低,心肌梗死范围缩小,SOD、GSH-PX活性升高,XOD活性降低,MDA含量减少。结论LIP具有与CIP相似的心脏保护作用,其机制与增强心肌抗氧化能力有关。
Objective To study the protective effect of noninvasive limb ischemic preconditioning on myocardial ischemia / reperfusion injury and to explore its mechanism from the perspective of oxidation-oxidation. Methods The rats were randomly divided into 3 groups: ischemia / reperfusion (I / R), ischemic preconditioning (CIP) and limb ischemic preconditioning (LIP). LIP group experienced left ischemic preconditioning for 3d consecutive days. d4, all groups of animals experienced myocardial ischemia / reperfusion injury, CIP group before ischemia ischemic preconditioning. The changes of electrocardiogram (ECG) were measured continuously. The extent of myocardial infarction was detected. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and xanthine oxidase (XOD) )content. Results Compared with I / R group, the amplitude of ST-segment elevation in LIP group and CIP group was lower than that in I / R group. The early onset of ventricular tachycardia was delayed, the duration was shortened, the incidence of ventricular arrhythmia was decreased, the infarct size was narrowed and the activity of SOD and GSH-PX High, XOD activity decreased, MDA content decreased. Conclusion LIP has cardioprotection similar to that of CIP, and its mechanism is related to the enhancement of myocardial antioxidant capacity.