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目的用SPSS和SAS程序计算小剂量激素诱导前后肾小球系膜细胞(HMC)对顺铂的IC50,进而计算耐药倍数。方法体外培养HMC,予小剂量(2μg/ml)甲基强的松龙刺激48h为耐药组,对照组常规培养,四甲基偶氮唑盐(MTT)法检测吸光度。应用SPSS和SAS程序计算IC50,计算经激素诱导后HMC对于顺铂的耐药倍数。结果 SPSS计算结果耐药倍数为5.388,SAS统计软件计算耐药倍数为5.382。结论 2种软件的计算结果比较接近且计算方便快捷,但SPSS计算更加快速、直观、方便。
Objective To calculate the IC50 of cisplatin on glomerular mesangial cells (HMC) before and after induction of small dose hormone by SPSS and SAS programs, and then calculate the drug resistance multiple. Methods HMC was cultured in vitro. The mice were challenged with low dose (2μg / ml) methylprednisolone for 48h and the control group were cultured routinely. The absorbance was measured by MTT assay. The IC50 was calculated using SPSS and SAS programs, and the resistance multiples of HMC to cisplatin after hormone induction were calculated. Results The result of SPSS showed that the multiple of drug resistance was 5.388, and SAS statistical software calculated the multiple of drug resistance of 5.382. Conclusion The calculation results of the two kinds of software are relatively close and the calculation is quick and easy, but SPSS calculation is more rapid, intuitive and convenient.